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rox  (Biotium)


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    Structured Review

    Biotium rox
    Rox, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rox+passive+reference+dye/ROX+Passive+Reference+Dye+(5x1mL)/pm40531297-56-38-39
    Average 93 stars, based on 13 article reviews
    rox - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Salt stress-induced Ca 2+ waves are associated with rapid, long-distance root-to-shoot signaling in plants
    Article Snippet: Total RNA (1–2 μg) was reverse transcribed into first-strand cDNA in a 40-μL reaction (25–50 ng of total RNA per μL) with the SuperScriptIII first-strand synthesis system for reverse transcription-PCR (Invitrogen). qPCR analysis was performed using a Stratagene Mx3000P QPCR System, and analysis was performed with the MxPro qPCR software (Stratagene). .. The Arabidopsis UBQ10 gene was used as an internal reference for standardization as described previously ( 35 ). cDNA proportional to 10 ng of starting total RNA was combined with 200 nM of each primer and 7.5 μL of 2× EvaGreen qPCR master mixed with ROX passive reference dye (Biotium) in a final volume of 15 μL. qPCRs were performed in a 96-well optical PCR plate (ABgene) using the following parameters: 1 cycle of 15 min at 95 °C, and 40 cycles of 20 s at 95 °C, 15 s at 58 °C, and 15 s at 65 °C, and 1 cycle of dissociation from 58 to 95 °C with 0.5° increments. ..

    Article Title: Variation in the transcriptome of different ecotypes of Arabidopsis thaliana reveals signatures of oxidative stress in plant responses to spaceflight.
    Article Snippet: .. Each reaction contained cDNA proportional to 10 ng of starting total RNA, 200 nM of primer, 7.5 μL of 2X EvaGreen qPCR master mix with ROX passive reference dye (Biotium, Hayward, CA, USA) made to a final volume of 15 μL with water. .. Quantitative PCR analysis was performed in 96- well optical PCR plates (ABgene, Epsom, UK) with one cycle of 15 min at 95°C; 40 cycles of 20 s at 95°C, 15 s at 58°C, and 15 s at 65°C; and one cycle of 58 to 95°C at 0.5° increments.

    Polymerase Chain Reaction:

    Article Title: Salt stress-induced Ca 2+ waves are associated with rapid, long-distance root-to-shoot signaling in plants
    Article Snippet: Total RNA (1–2 μg) was reverse transcribed into first-strand cDNA in a 40-μL reaction (25–50 ng of total RNA per μL) with the SuperScriptIII first-strand synthesis system for reverse transcription-PCR (Invitrogen). qPCR analysis was performed using a Stratagene Mx3000P QPCR System, and analysis was performed with the MxPro qPCR software (Stratagene). .. The Arabidopsis UBQ10 gene was used as an internal reference for standardization as described previously ( 35 ). cDNA proportional to 10 ng of starting total RNA was combined with 200 nM of each primer and 7.5 μL of 2× EvaGreen qPCR master mixed with ROX passive reference dye (Biotium) in a final volume of 15 μL. qPCRs were performed in a 96-well optical PCR plate (ABgene) using the following parameters: 1 cycle of 15 min at 95 °C, and 40 cycles of 20 s at 95 °C, 15 s at 58 °C, and 15 s at 65 °C, and 1 cycle of dissociation from 58 to 95 °C with 0.5° increments. ..



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